Berry List of Biology Experiments (PEKA) Form 4 (Part 4)

by BerryBerryTeacher

in Berry Experiment (Experiments & PEKA)

This post is the list of PEKA experiments for SPM Biology Form 4 from Berry Berry Easy. It is advisable that students read all of these experiments from Chapter 4: Chemical Composition of the Cell before heading in so as to be better prepared. So do read this post.

Berry List of Biology Experiments (PEKA) Form 4 (Part 4)

peka-spm-biology-chapter-4

Chapter 4 – Chemical Composition of the Cell

List of PEKA experiments:

  1. Differentiating between reducing and non-reducing sugars
  2. Conducting a role-play to show the involvement of specific organelles in the production of extracellular enzymes
  3. Studying the effects of temperature on salivary amylase activity
  4. Studying the effects of pH on the activity of pepsin
  5. Studying the effects of substrate concentration on salivary amylase activity
  6. Studying the effect of enzyme concentration on the activity of salivary amylase
  7. Conducting a role-play to show the consequences of deficiency in one of the chemical components in the cell
Number Activity 4.1 (Observation)
Pg. 33
Title Differentiating between reducing and non-reducing sugars
Aim / Objective of the Study To differentiate between reducing and non-reducing sugars
Material
  • Benedict’s solution
  • Dilute hydrochloric acid
  • Sodium hydrogen carbonate powder
  • 10% glucose solution
  • 10% sucrose solution
Apparatus
  • Test tubes
  • Test tube holder
  • Beaker
  • Dropper
  • Bunsen burner
  • Tripod
  • Wire gauze
Technique used Carry out testing for the presence of reducing sugars with Benedict’s solution

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Number Activity 4.2 (Simulation)
Pg. 35
Title Conducting a role-play to show the involvement of specific organelles in the production of extracellular enzymes
Aim / Objective of the Study To conduct a role-play to show the involvement of specific organelles in the production of extracellular enzymes
Material
  • Cardboard
  • Marker pen
  • Newspaper
  • Strings
Apparatus -
Technique used Conduct and involvement in a role-play to show the involvement of specific organelles in the production of extracellular enzymes

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Number Activity 4.3 (Experiment)
Pg. 36
Title Studying the effects of temperature on salivary amylase activity
Aim / Objective of the Study To study the effects of temperature on salivary amylase activity
Problem Statement What are the effects of temperature on salivary amylase activity?
Hypothesis The rate of reaction catalysed by salivary amylase is highest at 37˚C / The optimum temperature for salivary amylase is 37˚C
Variables
  • Manipulated variable: Temperature of medium of reaction
  • Responding variable: The rate of reaction catalysed by salivary amylase
  • Fixed variable: Volume of saliva, volume and concentration of starch suspension and pH of medium
Material
  • 1% starch suspension
  • Saliva suspension
  • Iodine solution
  • Ice cubes
  • Distilled water
Apparatus
  • Beakers
  • Test tubes
  • Test tube rack
  • Syringes
  • Droppers
  • Glass rods
  • White tile with grooves
  • Thermometer
  • Bunsen burner
  • Tripod stand
  • Wire gauze
  • Stopwatch
Technique used
  • Test the presence of starch using iodine test
  • Record the time taken for the hydrolysis of starch to be completed.

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Number Activity 4.4 (Experiment)
Pg. 39
Title Studying the effects of pH on the activity of pepsin
Aim / Objective of the Study To study the effects of pH on the activity of pepsin
Problem Statement What are the effects of pH on the activity of pepsin?
Hypothesis An acidic medium at pH 3 is optimum for the activity of pepsin
Variables
  • Manipulated variable: pH of medium
  • Responding variable: Rate of reaction catalysed by pepsin
  • Fixed variable: Volume and concentration of albumen suspension, volume and concentration of pepsin solution and temperature of medium
Material
  • Egg albumen suspension
  • 1% pepsin solution
  • 0.1 M hydrochloric acid
  • 0.1 M sodium hydroxide solution
  • Distilled water
Apparatus
  • Beakers
  • Test tubes
  • Test tube rack
  • Droppers
  • Glass rod
  • Thermometer
  • 5 ml syringes
  • pH paper
  • Bunsen burner
  • Tripod stand
  • Wire gauze
  • Stopwatch
Technique used Observe and record the conditions of mixtures before and after 20 minutes

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Number Activity 4.5 (Experiment)
Pg. 42
Title Studying the effects of substrate concentration on salivary amylase activity
Aim / Objective of the Study To study the effects of substrate concentration on salivary amylase activity
Problem Statement What are the effects of substrate concentration on salivary amylase activity?
Hypothesis The rate of enzymatic reaction increases with the increase in substrate concentration until it reaches a maximum rate
Variables
  • Manipulated variable: Substrate concentration / Concentration of starch suspension
  • Responding variable: Rate of reaction / Time taken for the hydrolysis of starch to be completed
  • Fixed variable: Enzyme concentration, pH of medium, volume of starch, volume of saliva suspension and surrounding temperature
Material
  • 0.1% starch suspension
  • 0.2% starch suspension
  • 0.3% starch suspension
  • 0.4% starch suspension
  • 0.5% starch suspension
  • 0.6 % starch suspension
  • 0.1% amylase solution / saliva suspension
  • Iodine solution
Apparatus
  • Syringes
  • White tiles with grooves
  • Test tubes
  • Glass rod
  • Dropper
  • Measuring cylinder
  • Stopwatch
  • Beaker
  • Thermometer
  • Water bath (Bunsen burner, tripod stand and wire gauze)
Technique used
  • Test for the presence of starch using iodine test
  • Record the time taken for the hydrolysis of starch to be completed with a stopwatch

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Number Activity 4.6 (Experiment)
Pg. 43
Title Studying the effect of enzyme concentration on the activity of salivary amylase
Aim / Objective of the Study To study the effect of enzyme concentration on the activity of salivary amylase
Problem Statement What are the effects of enzyme concentration on the activity of salivary amylase?
Hypothesis The rate of enzymatic reaction increases with the increase in enzyme concentration as long as there are no other factors limiting the rate of reaction.
Variables
  • Manipulated variable: Enzyme concentration
  • Responding variable: Rate of reaction
  • Fixed: Substrate concentration and temperature
Material
  • 1% starch suspension
  • 0.5% amylase solution / saliva suspension
  • Iodine solution
  • Distilled water
Apparatus
  • 5 ml of syringe
  • 1 ml of syringe
  • White tiles with grooves
  • Test tubes
  • Glass rod
  • Dropper
  • Measuring cylinder
  • Stopwatch
  • Beaker
  • Thermometer
  • Water bath (Bunsen burner, tripod stand and wire gauze)
Technique used
  • Test for the presence of starch using iodine test
  • Record the time taken for the hydrolysis of starch to be completed with a stopwatch

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Number Activity 4.7 (Simulation)
Pg. 45
Title Conducting a role-play to show the consequences of deficiency in one of the chemical components in the cell
Aim / Objective of the Study To study and predict the consequences of deficiency in carbohydrate, protein, lipids or enzymes in the cell.
Material
  • Cardboard
  • Marker pen
  • Strings
Apparatus -
Technique used Conduct and involvement in a role-play to show the consequences of deficiency in one of the chemical components in the cell

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